Axl Mouse anti-Human, PE, Clone: DS7HAXL, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 12108741
This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis. Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis. Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green LaserAxl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and auto-phosphorylation upon ligand binding. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (through SOCS-1 and SOCS-3), which limits TLR and cytokine signaling. This results in dampened inflammatory responses in macrophages and DCs. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
|ARK; AXL; JTK11; Tyro7; UFO|
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