Axygen™ AxyPrep Mag™ PCR Clean-up Kits Available on GSA/VA Contract for Federal Government customers only.

Post PCR clean-up simplified

Manufacturer: Axygen Scientific MAGPCRCL50

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Catalog No. 14-223-152

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    Specifications

    For Use With (Application) Efficient removal of unincorporated dNTPs, salts and Enzymes
    Format 96 and 384-well plate
    Product Type Kit
    Quantity 50mL

    The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of down stream applications such as genotyping Sanger and next generation sequencing. The AxyPrep Mag PCR Clean-up kit utilizes a unique paramagnetic bead technology for quick high-throughput purification of PCR amplicons. It also utilizes an optimized buffer to selectively bind PCR amplicons of variable sizes >60 bp or >100 bp to paramagnetic beads, providing end users flexibility and high recovery options with efficient primer-dimer removal based on their downstream applications needs. The protocol mainly consists of binding, washing and elution steps. Primers, nucleotides, salts and enzymes in PCR mixture are removed during the binding and washing steps. The purified PCR product is essentially free of contaminants.

    • Flexible clean-up: high recovery of amplicons with the option to choose recovery of >60 bp or >100 bp
    • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
    • Manual and automation friendly protocol
    • Processing time: 15 minutes/96 samples
    • No centrifugation or filtration required
    • Scalable: tube, 96 and 384 well plate formats

    Applications:

    PCR, Sequencing (Sanger and Next Generation), Genotyping and SNP detection, Restriction enzyme clean-up, Cloning

  • Description & Specifications

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