Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit

Description

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents.

These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).

Highlights:

• Ready to use – B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations

• Fast and simple – just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris

• Excellent yields – recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels

• Flexible – B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes

• Compatible – completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications

Convenient, ready-to-use formats:

• B-PER and B-PER II (2X) Reagents are free of enzymatic components
• B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking
• B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery

Includes:

Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Specifications

• Quantity: 500 mL
• Format: Liquid
• Product Type: Bacterial Protein Extraction Kit
• Volume (Metric): 500 mL
• Content And Storage: Sufficient For: 125g cell paste
  • B-PER Reagent, 500 mL
  • Lysozyme, 50 mg/mL, 2 x 0.5 mL
  • DNase I, 2500 U/mL, 2 x 0.5 mL
  
  Store B-PER Reagent at ambient temperature. Store Lysozyme and DNaseI at -20°C.
• Product Line: B-PER™

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.