Novus Biologicals™ B7-H3/CD276 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human B7-H3/CD276.

Specifications

• Assay Range: 9.38 pg/mL
• Assay Sensitivity: 9.38-600 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human B7-H3/CD276.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276 antigen, CD276 molecule, Costimulatory molecule
• Gene ID (Entrez): 80381
• Gene Symbol: CD276
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 25 ng of recombinant Human B7-H3/CD276.
  Capture Antibody: 0.2 mg/mL of mouse anti-Human B7-H3/CD276 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of mouse anti-Human B7-H3/CD276 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: B7-H3/CD276
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.