Invitrogen™ B7-H4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA587879

Catalog No. PIPA587879

Description

Immunogen sequence: RTAVFADQVI VGNASLRLKN VQLTDAGTYK CYIITSKGKG NANLEYKTGA FSMPEVNVDY NASSETLRCE APRWFPQPTV VWASQVDQGA NFSEVSNTSF ELNSENVTMK VVSVLYNVTI NNTYSCMIEN DIAKATGDIK VTESEIKRRS HLQLLNSKAS L; Positive Samples: MCF-7, SKOV3, HeLa, 293T, B-cell, Mouse uterus, Rat thymus; Cellular Location: Cell membrane, Single-pass type I membrane protein.

B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).

Specifications

• Antigen: B7-H4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Vtcn1
• Gene Alias: B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7h.5; B7H4; B7-H4; B7s1; B7x; BC032925; EMBL:AAH95842.1}; FLJ22418; immune costimulatory protein B7-H4; PRO1291; Protein B7S1; RGD1311204; RP11-229A19.4; t cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; UNQ659/PRO1291; VCTN1; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1; vtcn1 {ECO:0000312
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 242122, 295322, 79679
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 2.91 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q501W4, Q7TSP5, Q7Z7D3
• Gene Symbols: Vtcn1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human B7-H4/VTCN1 (NP_0789022).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov