Invitrogen™ B7-H6 Monoclonal Antibody (JAM1EW), Super Bright™ 436, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
62-652-642	Invitrogen™ 62652642	100 Tests

Description

This JAM1EW monoclonal antibody recognizes human B7-H6 (NCR3LG1) protein on the surface of cells. It recognizes protein on live but not on paraformaldehyde-fixed cells. The JAM1EW clone is specific to B7-H6. When tested in flow cytometry against cells transfected with either B7-H4, B7-H5, B7-H6, or B7-H7, clone JAM1EW only stains positively on B7-H6 transfected cells. Applications Reported: This JAM1EW antibody has been reported for use in flow cytometric analysis. Applications Tested: This JAM1EW antibody has been pre-diluted and tested by flow cytometric analysis of the HL-60 cell line. This may be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton...

B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.

Specifications

• Antigen: B7-H6
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q68D85
• Gene ID (Entrez): 374383
• Immunogen: B7-H6 recombinant protein.
• Product Type: Antibody

• Clone: JAM1EW
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: NCR3LG1
• Gene Alias: B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; NCR3LG1; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
• Gene Symbols: NCR3LG1
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography