Invitrogen™ Human BACE1 (beta-Secretase) Recombinant Protein

Catalog No.	Quantity
P2947	5 units

Catalog No. P2947 Supplier Invitrogen™ Supplier No. P2947

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a Swedish mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Specifications

• Accession Number: P56817
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: Proprietary buffer with no preservative
• Gene ID (Entrez): 23621
• Molecular Weight (g/mol): 55 kDa
• Name: Human BACE1 (beta-Secretase)
• Purification Method: Purified
• Quantity: 5 units
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE; BACE 1; BACE1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; C76936; HSPC104; hypothetical protein LOC403005; KIAA1149; Memapsin; Memapsin2; Memapsin-2; membrane-associated aspartic protease 2; protease; transmembrane aspartic proteinase Asp2; zgc:77409
• Common Name: BACE1
• Gene Symbol: BACE1
• Biological Activity: 609 Units/mg One unit is defined as the amount of BACE1 required to cleave one pmol of Rhodamine-EVNLDAEFK-Quencher in one minute (at room temperature. Cleavage of the FRET peptide is monitored by an increase in fluorescence (E x 535 nm/ Em590 nm) in the following assay reaction: 250 nM Rhodamine-EVNLDAEFK-Quencher in 50 mM Sodium Acetate (pH 4.5) in a total volume of 30 μL accoding to the BACE1 (βSecretase) FRET Assay Kit, Red (P2985).)
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: TQHGIRLPLR
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Activity: 609 Units/mg. One unit is defined as the amount of BACE1 required to cleave one pmol of Rhodamine-EVNLDAEFK-Quencher in one minute at room temperature. Cleavage of the FRET peptide is monitored by an increase in fluorescence (Ex535 nm/ Em590 nm) in the following assay reaction: 250 nM Rhodamine-EVNLDAEFK-Quencher in 50 mM Sodium Acetate (pH 4.5) in a total volume of 30 μL accoding to the BACE1 (ßSecretase) FRET Assay Kit, Red (P2985).
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Form: Liquid
• Protein Subtype: Amyloid-Related Proteins
• Research Category: Neurobiology
• Purity or Quality Grade: 85% as determined by a Coomassie™ blue stained SDS-PAGE gel.