Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 17699180

Catalog No. 50-152-94

Description

Description: The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV). The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection. The AcV1 antibody can be used in flow cytometry-based viral titration experiments. Applications Reported: This AcV1 antibody has been reported for use in flow cytometric analysis. This antibody can be used in identifying virally-infected insect cells and determining viral titers. Applications Tested: This AcV1 antibody has been tested flow cytometric analysis of baculovirus-infected insect cells. This can be used at less than or equal to 0.015 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Baculoviruses are known to infect invertebrates, with over 600 species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells. The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection.

Specifications

• Antigen: Baculovirus Envelope gp64
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: AcV1
• Conjugate: APC
• Gene Alias: BV gp64
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgG2a

For Research Use Only.