Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. BAF180, Rat anti-Human, Clone: 370.1.8C3 , Millipore Sigma™

BAF180, Rat anti-Human, Clone: 370.1.8C3 , Millipore Sigma™

Catalog No. MABE1138100 Shop All MilliporeSigma Products
Change view
Click to view available options
Quantity:
25 μL
100 μL
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
MABE1138100 100 μL
MABE113825 25 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. MABE1138100 Supplier MilliporeSigma Supplier No. MABE1138100UL
Add to Cart
Add to Cart

Description

Rat Monoclonal Antibody

Anti-BAF180, clone 370.1.8C3, Cat. No. MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting.

Protein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a large, chromatin-remodeling protein that contains six bromodomains, two BAH-domains, and one HMG-box. Bromodomains recognize and bind to acetylated lysines, the BAH (bromo-adjacent homology) domains mediate protein protein interactions, and the HMG-box domain binds to DNA. Nine isoforms of BAF180 have been described that are produced by alternative splicing. BAF180 serves as a subunit of the SWI/SNF complex and plays critical roles in the regulation of chromatin remodeling and gene transcription, and is reported to be frequently mutated in several human cancers. BAF180 acts asa negative regulator of cell proliferation and plays a role in suppression of tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter. Mutations in PBRM1 gene are known to cause renal cell carcinoma. (Ref.: Lee, H., et al. (2016). Oncotarget 7(15);19134-46; Gao, W., et al. (2017). Proc. Natl. Acad. Sci. USA. 114(5):1027-1032).
TRUSTED_SUSTAINABILITY

Specifications

Antigen BAF180
Applications Western Blot
Classification Monoclonal
Clone 370.1.8C3
Conjugate Unconjugated
Dilution Western Blotting Analysis: A representative lot detected BAF180 in Western Blotting applications (Gao, W., et. al. (2017). Proc Natl Acad Sci U S A. 114(5):1027-1032).
Formulation Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Alias Protein polybromo-1;hPB1;BRG1-associated factor 180;Polybromo-1D
Gene Symbols PBRM1;BAF180;PB1
Host Species Rat
Immunogen GST-tagged recombinant fragment corresponding to 241 amino acids from the C-terminal half of human Protein polybromo-1 (BAF180).
Purification Method Protein G purified
Quantity 100 μL
Regulatory Status RUO
Research Discipline Epigenetics & Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_060783.3
Test Specificity Clone 370.1.8C3 is a rat monoclonal antibody that specifically detects human Protein Polybromo-1 (BAF180). It targets an epitope within 241 amino acids from the C-terminal half.
Target Species Human
Content And Storage Stable for 1 year at 2-8°C from date of receipt.
Form Purified
Isotype IgG2a κ
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.