Invitrogen™ BAF53B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5104421

Catalog No. PIPA5104421

Description

Antibody detects endogenous levels of total ACTL6B.

Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.

Specifications

• Antigen: BAF53B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Actl6b
• Gene Alias: 53 kDa BRG1-associated factor A; 53 kDa BRG1-associated factor B; actin like 6A; actin like 6B; actin-like 6; actin-like 6A; actin-like 6B; actin-like protein 6A; Actin-like protein 6B; actin-like protein 6B {ECO:0000250; actin-related protein 4; actin-related protein Baf53a; Actin-related protein Baf53b; ACTL6; ACTL6A; Actl6b; actl6b {ECO:0000250; Arp4; ArpNa; ArpNalpha; ArpN-alpha; arpNbeta; ARPN-BETA; BAF complex 53 kDa subunit; BAF53; BAF53A; BAF53B; BRG1-associated factor 53A; BRG1-associated factor 53B; hArpN alpha; hArpN beta; INO80 complex subunit K; INO80K; UniProtKB:O94805}
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51412, 83766
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O94805, Q99MR0
• Gene Symbols: Actl6b
• Immunogen: A synthesized peptide derived from human ACTL6B(Accession O94805), corresponding to amino acid residues V266-G316.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov