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Invitrogen™ BAFF Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P1631MG

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Catalog No. 500P1631MG


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Description

Description

AA Sequence of recombinant protein: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography employing an immobilized Human BAFF matrix. Sandwich ELISA: To detect Human BAFF by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BAFF (500-P163BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human BAFF. Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

BAFF
Polyclonal
Unconjugated
TNFSF13B
ApoL related ligand TALL-1; b cell-activating factor; B lymphocyte stimulator; BAFF; B-cell-activating factor; B-lymphocyte stimulator; BLYS; CD257; D8Ertd387e; delta BAFF; Delta4 BAFF; dendritic cell-derived TNF-like molecule; DTL; RGD1561519; TALL1; TALL-1; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; Tnfsf13b; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4; UNQ401/PRO738; ZTNF4
Rabbit
Antigen Affinity Chromatography
RUO
10673
-20°C
Lyophilized
ELISA, Western Blot
0.1-1.0 mg/mL
PBS with no preservative
Q9Y275
TNFSF13B
E.coli-derived Recombinant Human BAFF.
1 mg
Primary
Human
Antibody
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