Invitrogen™ Cytokeratin Pan Type II Monoclonal Antibody (AE3), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 14900082

Catalog No. 50-137-61

Description

Description: The monoclonal antibody AE3 recognizes many members of the basic (Type II) cytokeratin family. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm. The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins (also known as cytokeratin 1, 2, 3, 4, 5, 6, 7 and 8) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken. Applications Reported: This AE3 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This AE3 antibody has been by tested western blot on SDS-reduced lysates from MCF7 cells at less than or equal to 2 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Specifications

• Antigen: Cytokeratin Pan Type II
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O93532, P02538, P04104, P04264, P05787, P07744, P08729, P11679, P12035, P13647, P19013, P35908, P50446, Q01546, Q14CN4, Q29426, Q3TTY5, Q3UV17, Q6EIY9, Q6EIZ1, Q6IME9, Q922U2, Q9DCV7
• Gene Symbols: KRT1, KRT2, KRT3, KRT4, KRT5, Krt6a, KRT7, KRT72, KRT76, KRT8
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100345579, 100348388, 100348891, 100352658, 100353162, 100353415, 100353668, 100424400, 100425096, 100682832, 100683401, 100687760, 105866, 110308, 110310, 140807, 16678, 16681, 16682, 16687, 16691, 3848, 3849, 3850, 3851, 3852, 3853, 3855, 3856, 395772, 407779, 408041, 426896, 444850, 444857, 477602, 486513, 486518, 486520, 486523, 51350, 697425, 699194, 699320, 699567, 700060, 700449, 705390, 718960, 768882, 769040, 77055
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: AE3
• Conjugate: Unconjugated
• Gene: KRT7
• Gene Alias: 2310016L08Rik; 3300001P10Rik; 57kd keratin; 57kDa keratin; 58 kda cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; AA960620; AI507495; AL022697; alpha keratin; AU019895; AW108092; AW146334; BB005427; Card2; CK 2e; CK1; CK-1; CK-2e; CK3; CK-3; CK4; CK-4; CK5; CK-5; CK6A; CK-6A; CK6C; CK-6C; CK6D; CK-6D; CK-6E; CK7; CK-7; CK-72; CK8; CK-8; CYK4; CYK8; CYKER; cytokeratin 1; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; cytokeratin 7; cytokeratin 8; cytokeratin 8 (370 AA); cytokeratin endo A; Cytokeratin otokeratin; cytokeratin type II, component Ib/c; cytokeratin type II, component III; Cytokeratin-1; Cytokeratin-2e; cytokeratin-3; Cytokeratin-4; cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; cytokeratin-7; Cytokeratin-72; cytokeratin-8; Cytokeratin-A; cytoskeletal 57 kDa keratin; D15Wsu77e; DDD; DDD1; ear specific cytokeratin; EBS2; EGK_03684; EGK_03685; EHK; EHK1; EndoA; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; epithelial keratin 1; epithelial keratin 2e; Epithelial keratin-1; Epithelial keratin-2e; EPPK; Hair alpha protein; Hom s 5; K1; K2C7; K2C8; K2E; K3; K3 keratin; K4; K5; K6A; K6a keratin; K6C; K6D; K6irs; K6IRS2; K7; K72; K8; kamp-keratin derived antimicrobial peptide; Kb1; Kb2; Kb35; Kb4; Kb7; KDAMP; KER1; Ker2; KERA; keratin; keratin 1; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 2; keratin 2 (epidermal ichthyosis bullosa of Siemens); keratin 2 epidermis; keratin 2, type II; keratin 2A; keratin 2A (epidermal ichthyosis bullosa of Siemens); keratin 3; keratin 3, type II; keratin 4; keratin 4, type II; keratin 5; keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6 alpha; keratin 6, inner root sheath, 2; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 7; keratin 7, type II; keratin 72; keratin 72, pseudogene; keratin 72, type II; keratin 8; keratin 8, type II; keratin 86; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 35; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6a; keratin IV; keratin K3; keratin K6; keratin K6h; keratin protein K6irs; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 59 kDa, component IV; keratin, type II cytoskeletal 60 kDa, component III; keratin, type II cytoskeletal 6A; keratin, type II cytoskeletal 6C; keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 72; keratin, type II cytoskeletal 8; Keratin, type II cytoskeletal cochleal; keratin-1; Keratin-2 epidermis; keratin-2e; Keratin-3; Keratin-4; keratin-5; keratin-6A; Keratin-6-alpha; keratin-6C; keratin-7; Keratin-72; keratin-8; KERIB; KO; Krt1; krt1 {ECO:0000250; KRT1A; Krt2; krt2 {ECO:0000250; Krt-2.1; Krt-2.4; Krt-2.8; Krt2-1; Krt2-17; Krt2-2; Krt2-35; Krt2-4; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-7; KRT2-8; KRT2A; KRT2E; KRT3; KRT4; krt4 {ECO:0000250; Krt5; KRT5A; KRT6; Krt6a; KRT6C; KRT6D; KRT6IRS2; Krt7; krt7 {ECO:0000250; Krt72; Krt72-ps; Krt8; Krt86; KRTA; KRTE; LOC683313; LOW QUALITY PROTEIN: keratin, type II cytoskeletal 7; MGC116967; MGC116968; mK6[a]; MK6a; mK6-alpha; NEPPK; otokeratin; pan keratin; PC3; Sak57; sarcolectin; SCL; similar to keratin 7; similar to keratin complex 2, basic, gene 6a; Tfip8; tuftelin interacting protein 8
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Canine, Rabbit, Monkey, Chicken, Rhesus Macaque
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.