Bcl-2 Antibody (BCL2/2210R) - Azide and BSA Free, Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP275764

Catalog No. NBP275764

Description

Bcl-2 Monoclonal specifically detects Bcl-2 in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: Bcl-2
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot 0.5 - 1 μg/mL, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1 - 2 μg/mL, Immunohistochemistry-Paraffin 0.5 - 1 μg/mL, Protein Array
• Gene Accession No.: P10415, P10415
• Gene Symbols: BCL2
• Immunogen: Recombinant full-length human bcl-2 protein (Uniprot: P10415)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
• Content And Storage: Store at 4°C.
• Isotype: IgG

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Peptide Array
• Clone: BCL2/2210R
• Conjugate: Unconjugated
• Formulation: 10 mM PBS with No Preservative
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Host Species: Rabbit
• Purification Method: Protein A or G purified
• Research Discipline: Apoptosis, Autophagy, Biologically Active Proteins, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Oncogenes, Tumor Suppressors
• Gene ID (Entrez): 596.0
• Target Species: Human
• Form: Purified