Invitrogen™ Bcl-2 Monoclonal Antibody (8E12)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515668

Catalog No. PIMA515668

Description

MA5-15668 targets BCL-2 in indirect ELISA, FACS, IF, and IHC applications and shows reactivity with Human and mouse samples. The MA5-15668 immunogen is synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH. MA5-15668 detects BCL-2 which has a predicted molecular weight of approximately 26kDa.

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Specifications

• Antigen: Bcl-2
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.03% sodium azide
• Gene Accession No.: P10415, P10417
• Gene Symbols: Bcl2
• Immunogen: Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.
• Regulatory Status: RUO
• Gene ID (Entrez): 12043, 596
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: 8E12
• Conjugate: Unconjugated
• Gene: Bcl2
• Gene Alias: apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1