Invitrogen™ Bcl-2 Monoclonal Antibody (Bcl-2/100), eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ BMS1028

Catalog No. 50-176-91

Description

Description: Bcl-2 is a key regulator of apoptosis and the founding member of Bcl-2 family of apoptosis regulatory protiens. The anti-apoptotic functions of Bcl-2 is achieved by activation or inactivation of an inner mitochondrial permeability transition pore, which is involved in the regulation of matrix Ca2+, pH, and voltage. Applications Tested: ELISA, Flow Cytometry, Immunohistochemistry, Western Blotting.

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Specifications

• Antigen: Bcl-2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2-7.4
• Gene Accession No.: P10415
• Gene Symbols: Bcl2
• Immunogen: Rec. Human Bcl-2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
• Clone: Bcl-2/100
• Conjugate: Unconjugated
• Gene: Bcl2
• Gene Alias: apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 596
• Content And Storage: -20°C
• Form: Liquid