Bcl-2 Mouse anti-Human, Mouse, Rat, Alexa Fluor™ 750, Clone: 100/D5+124, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234515AF750

Catalog No. NP2341A70

Description

Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Bcl-2
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: BCL2
• Immunogen: A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 (100/D5 & 124) (Uniprot: P10415)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 100/D5+124
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Apoptosis, Autophagy, Biologically Active Proteins, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Oncogenes, Phospho Specific, Tumor Suppressors
• Gene ID (Entrez): 596.0
• Target Species: Human, Mouse, Rat
• Isotype: IgG1 κ