Bcl-2 Mouse, Clone: SPM117, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234391F	Novus Biologicals NBP2343910.02MG	0.02 mg
NBP234391A	Novus Biologicals NBP2343910.1MG	0.1 mg
NBP234391B	Novus Biologicals NBP2343910.2MG	0.2 mg

Description

Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Bcl-2
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Gene Accession No.: P10415
• Gene ID (Entrez): 596
• Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 25 kDa

• Clone: SPM117
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Gene Symbols: BCL2
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.