Invitrogen™ Bcl-X Monoclonal Antibody (2H12), eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-135-73	Invitrogen™ 14699481	50 μg

Description

Description: The 2H12 antibody reacts with human, mouse and rat Bcl-X. Bcl-X functions as a central repressor of several apoptotic signals.This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. Applications Reported: The 2H12 antibody has been reported for use in immunoblotting (WB). Applications Tested: The 2H12 antibody has been tested by Western blot analysis of HeLa lysate and can be used at less than 2 μg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.

Specifications

• Antigen: Bcl-X
• Concentration: 0.5 mg/mL
• Applications: Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P53563, Q07817, Q64373
• Gene ID (Entrez): 12048, 24888, 598
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 2H12
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human, Mouse, Rat, Monkey
• Gene: BCL2L1
• Gene Alias: anti-apoptosis regulatory protein; anti-apoptotic Bcl-2 gene family member; anti-apoptotic Bcl-xL; anti-apoptotic regulator Bcl-xL; apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; Bcl(X)L; bcl-2 L1; BCL2 like 1; BCL2L; Bcl2l1; bcl2-L-1; BCL2-like 1; Bcl-2-like protein 1; BCL2-like protein 1; BCLX; bcl-x; Bcl-x long protein; BclxL; Bcl-xL; BCL-XL/S; BclXS; bcl-xS; BLC2L; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3; unnamed protein product
• Gene Symbols: BCL2L1
• Product Type: Antibody