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Bcl-X Monoclonal Antibody (2H12), Invitrogen™

Catalog No. PIMA514011
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500 μL
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PIMA514011 500 μL
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Catalog No. PIMA514011 Supplier Thermo Scientific Supplier No. MA514011
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Description

Mouse Monoclonal Antibody

This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice. Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.

The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Bcl-X
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Classification Monoclonal
Clone 2H12
Concentration 0.2 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
Gene BCL2L1
Gene Accession No. O77737, P53563, Q07817, Q64373
Gene Alias anti-apoptosis regulatory protein; anti-apoptotic Bcl-2 gene family member; anti-apoptotic Bcl-xL; anti-apoptotic regulator Bcl-xL; apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; Bcl(X)L; bcl-2 L1; BCL2 like 1; BCL2L; Bcl2l1; bcl2-L-1; BCL2-like 1; Bcl-2-like protein 1; BCL2-like protein 1; BCLX; bcl-x; Bcl-x long protein; BclxL; Bcl-xL; BCL-XL/S; BclXS; bcl-xS; BLC2L; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3; unnamed protein product
Gene Symbols BCL2L1
Host Species Mouse
Immunogen A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. This amino acid sequence is shared by human and murine bcl-X protein.
Purification Method Protein A
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 12048, 24888, 397536, 598
Target Species Human, Mouse, Pig, Rat
Content And Storage 4° C
Form Liquid
Isotype IgG2a
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