Invitrogen™ BCL6 Monoclonal Antibody (GI191E), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 14988737

Catalog No. 14-988-737

Description

Description: This GI191E monoclonal antibody reacts with mouse and human Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1. Applications Reported: This GI191E antibody has been reported for use in immunoblotting (WB) and immunohistochemistry (FFPE). Applications Tested: This GI191E antibody has been tested by immunoblotting of reduced cell lysate prepared from A20 cells and by immunohistochemistry on human tissue using high pH antigen retrieval. This antibody can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Specifications

• Antigen: BCL6
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P41182, P41183
• Gene Symbols: BCL6
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12053, 604
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: GI191E
• Conjugate: Unconjugated
• Gene: BCL6
• Gene Alias: B cell CLL/lymphoma 6; B cell leukemia/lymphoma 6; B-cell CLL/lymphoma 6; B-cell CLL/lymphoma 6 (zinc finger protein 51); B-cell leukemia/lymphoma 5; B-cell leukemia/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein; B-cell lymphoma 6 protein homolog; B-cell lymphoma 6 protein transcript; BCL5; BCL-5; Bcl6; Bcl-6; BCL6 transcription repressor; BCL6A; cys his2 zinc finger transcription factor; cys-his2 zinc finger transcription factor; LAZ3; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); lymphoma-associated zinc finger gene on chromosome 3; protein LAZ-3; ZBTB27; zinc finger and BTB domain-containing protein 27; Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc finger protein 51; Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S; ZNF51
• Host Species: Mouse
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1 κ