Invitrogen™ BDNF Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA118363

Catalog No. 01-670-615

Description

A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human. Use at 1-10 μg/mL for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 μg/mL (ED50). Reconstitute with 500 μL of distilled water. After reconstitution keep aliquots at -20º for a higher stability, and at 4ºC with an appropriate antibacterial agent.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Antigen: BDNF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BDNF
• Gene Alias: abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
• Host Species: Sheep
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 24225, 627
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
• Form: Lyophilized

• Applications: ELISA, Immunohistochemistry (Frozen), Neutralization
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P23363, P23560
• Gene Symbols: BDNF
• Immunogen: Recombinant human BDNF (aa 129-247).
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG