Invitrogen™ beta-1,2 Adaptin Monoclonal Antibody (100/1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA125065

Catalog No. MA125065

Description

Recommended positive controls: rat brain, MDCK. This antibody stains clathrin-coated membranes at the plasma membrane, and in the Golgi region of methanol-fixed MDBK cells. By Western bloy on bovine and rat brain preparations, this antibody detects a major band of AP-1 at ~115 kDa and a minor component at ~110 kDa, and bands at ~104 and ~112 kDa representing AP2. The antibody reacts with polypeptides at ~100 kDa in bovine liver, human heart fibroblasts and MDBK cells, but not with any components in the 110-115 kDa range from these sources, or from rat PC12 cells, neuroblastoma or astrocytes.

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: beta-1,2 Adaptin
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.1% sodium azide
• Gene Accession No.: P52303, P62944, P63010, Q08DS7, Q10567
• Gene Symbols: AP1B1, AP2B1
• Immunogen: AP2 adaptor from bovine brain.
• Regulatory Status: RUO
• Gene ID (Entrez): 140670, 162, 163, 282183, 29663, 506192
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 100/1
• Conjugate: Unconjugated
• Gene: AP1B1
• Gene Alias: adapter-related protein complex 1 subunit beta-1; adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptin, beta 2 (beta); adaptor protein complex AP-1 subunit beta-1; adaptor protein complex AP-1, beta 1 subunit; adaptor protein complex AP-2 subunit beta; adaptor related protein complex 1 beta 1 subunit; adaptor related protein complex 1 subunit beta 1; adaptor related protein complex 2 beta 1 subunit; adaptor related protein complex 2 subunit beta 1; adaptor related protein complex 2, beta 1 subunit; adaptor-related protein complex 1 beta 1 subunit; adaptor-related protein complex 1 subunit beta-1; adaptor-related protein complex 1, beta 1 subunit; adaptor-related protein complex 2 subunit beta; adaptor-related protein complex 2, beta 1 subunit; adaptor-related protein complex AP-1 beta 1 subunit; ADTB1; ADTB2; AP-1 complex subunit beta-1; AP105A; AP105B; AP1B1; AP-2 complex subunit beta; AP2B1; AP2-BETA; BAM22; beta adaptin; beta-1-adaptin; beta1-adaptin; Beta-2-adaptin; beta2-adaptin; beta-adaptin; Beta-adaptin 1; beta-prime-adaptin; Clapb1; CLAPB2; Clathrin assembly protein complex 1 beta large chain; clathrin assembly protein complex 2 beta large chain; clathrin-associated / assembly / adaptor protein, large, beta 1; clathrin-associated/assembly/adaptor protein, large, beta 1; golgi adaptor HA1/AP1 adaptin beta subunit; MGC128441 protein; plasma membrane adaptor HA2/AP2 adaptin beta subunit; plasma membrane adaptor HA2/AP2 adaptor beta subunit; testicular tissue protein Li 22
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat, Bovine
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov