beta-2-Microglobulin Polyclonal Antibody, HRP, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200403141

Catalog No. 50-199-0965

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine). Cross reactivity against b2-Microglobulin from other sources is unknown.

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

Specifications

• Antigen: beta-2-Microglobulin
• Classification: Polyclonal
• Conjugate: HRP
• Gene: B2m
• Gene Alias: B2M; B2-microglobulin; b2m-W01; b2m-W03; b2m-Z01; b2m-Z02; b2m-Z03; beta 2-microglobulin; beta chain of MHC class I molecules; Beta2 microglobulin; beta-2 microglobulin; beta2m; beta2-m; beta-2-microglobin; Beta-2-microglobulin; beta-2-microglobulin C-terminal fragment (55 AA); Beta-2-microglobulin form pI 5.3; B-F; CDABP0092; HDCMA22P; IMD43; Lactollin; Ly-m11; MHC Class I; Orla-B2m
• Host Species: Rabbit
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% gentamicin sulfate; pH 7.2
• Gene Accession No.: P61769
• Gene Symbols: B2m
• Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
• Regulatory Status: RUO
• Gene ID (Entrez): 567
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized