Invitrogen™ beta-3 Tubulin Monoclonal Antibody (2G10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1118X

Catalog No. PIMA1118X

Description

MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons. MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells. Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Specifications

• Antigen: beta-3 Tubulin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: Q13509, Q2T9S0, Q4QRB4, Q9ERD7
• Gene Symbols: LOC100341581, TUBB3
• Immunogen: A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin.
• Quantity: 20 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Bovine, Hamster, Pig, Guinea Pig
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 2G10
• Conjugate: Unconjugated
• Gene: TUBB3
• Gene Alias: 3200002H15Rik; beta 3 tubulin; beta tubulin 3; beta-4; betaIII-tubulin; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; I79_000194; LOC100341581; M(beta)3; M(beta)6; mc1R; melanocortin receptor 1; Neuron specific beta III Tubulin; neuron-specific class III beta-tubulin; TUBB; Tubb3; TUBB4; tubulin beta; tubulin beta 3 class III; Tubulin beta-3; tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4; Unknown (protein for MGC:134115)
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100341581, 100732882, 100760055, 10381, 106509340, 22152, 246118, 768070
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov