beta-Actin Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 13 publications

Supplier:  Novus Biologicals NB600505

Catalog No. NB600505

Description

beta-Actin Polyclonal specifically detects beta-Actin in Human, Mouse, Rat, Amphibian samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Electron Microscopy.

Specifications

• Antigen: beta-Actin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: ACTB, actin, beta, actin, cytoplasmic 1, B-Actin, Beta Actin, beta cytoskeletal actin, Beta-actin, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1
• Host Species: Rabbit
• Molecular Weight of Antigen: 42 kDa
• Quantity: 0.1 mg
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Loading Controls, Signal Transduction, Stem Cell Markers
• Gene ID (Entrez): 60
• Target Species: Human, Mouse, Rat, Amphibian, Bovine, Guinea Pig, Goat, Hamster, Sheep
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoassay
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:1000-1:4000, ELISA 1:10000-1:40000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry-Paraffin 1:50 - 1:200, Electron Microscopy
• Gene Accession No.: P60709
• Gene Symbols: ACTB
• Immunogen: beta-Actin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin. (Uniprot: P60709)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Expected to cross-react with a wide range of species including most vertebrates and some yeast due to sequence identity.
• Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.