Invitrogen™ beta Adducin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529499

Catalog No. PIPA529499

Description

Recommended positive controls: mouse brain. Predicted reactivity: Mouse (96%), Rat (95%), Rhesus Monkey (100%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.

Specifications

• Antigen: beta Adducin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Add2
• Gene Alias: 2900072M03Rik; ADD2; Add97; ADDB; adducin 2; adducin 2 (beta); adducin beta; adducin-63; beta-ADD; Beta-adducin; erythrocyte adducin subunit beta; snoRNA MBII-396
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11519, 119
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.92 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P35612, Q9QYB8
• Gene Symbols: Add2
• Immunogen: Recombinant protein encompassing a sequence within the center region of human beta Adducin. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov