Invitrogen™ beta Arrestin 1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5119643

Catalog No. PIPA5119643

Description

Positive Control: Human placenta tissue lysate, A549, HUVEC, LOVO, rat brain tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, Jurkat. Subcellular Location: Cytoplasm. Membrane. Nucleus.

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.

Specifications

• Antigen: beta Arrestin 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARRB1
• Gene Alias: 1200006I17Rik; ARB1; ARR1; ARRB1; ARRB1 protein; arrdc1; arrestin; arrestin 2; arrestin beta 1; arrestin beta-1; arrestin, beta 1; arrestin-2; AW208571; BARRES; beta-arrestin; beta-arrestin1; beta-arrestin-1; G430100A01Rik; Non-visual arrestin-2
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 109689, 25387, 408
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P29066, P49407, Q8BWG8
• Gene Symbols: ARRB1
• Immunogen: Synthetic peptide within N-terminal human Beta-Arrestin 1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG