Beta-Arrestin 1 Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A303745A

Catalog No. 50-156-5943

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.

Specifications

• Antigen: Beta-Arrestin 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARRB1
• Gene Alias: BARRES
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 408
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/ml
• Formulation: phosphate, tris citrate with 0.09% sodium azide; pH 7-8
• Gene Accession No.: P49407
• Gene Symbols: ARRB1
• Immunogen: Between 368 and 418
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG