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Thermo Scientific™ beta-Galactosidase Assay Stop Solution
Description
The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):
- Choose stand-alone assay reagent or complete kit for cell lysis and detection
- Colorimetric assay easily measured in a plate reader or spectrophotometer (405 nm)
- Adaptable to 96-well microplates or tube assay formats
- Perform homogeneous screening assays or measure activity secondarily after cell lysis
- Also compatiible with prepared lysates from other species such as bacteria
Related Products
- Mammalian beta-Galactosidase Assay Kit (Cat. No. 75707)
- beta-Galactosidase Assay Reagent (Cat. No. 75705)
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Detection Method | Reporter Enzyme |
| For Use With (Application) | Absorbance |
| For Use With (Equipment) | Microplate Reader |
| Product Type | Beta-Galactosidase Assay Stop Solution |
| Dye Type | Enzyme Labeled |
| Format | 96-well Plate |
| Product Line | Pierce |
| Quantity | 25 mL |
| Substrate | Proprietary |
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For Research Use Only. Not for use in diagnostic procedures.
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