Invitrogen™ beta Galactosidase Fusion Protein Polyclonal Antibody, eBioscience™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 14677381

Catalog No. 50-135-43

Description

Description: The rabbit polyclonal antibody reacts with bBeta galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope. Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining. Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.

Specifications

• Antigen: beta Galactosidase Fusion Protein
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: acid beta-galactosidase; AW125515; betagal; Beta-Gal; Beta-galactosidase; bgal; Bge; Bgl; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik; EBP; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; GLB1; Glb-1; lactase; lysosmal hydrolase; lysosomal beta-galactosidase; lysosomal hydrolase; MPS4B; ßgal; ß-gal; ß-galactosidase
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with BSA, 0.02% gelatin and 0.1% sodium azide
• Host Species: Rabbit
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid