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Invitrogen™ Beta-Lactamase (TEM-1) Recombinant Protein
Description
TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nitrocefin and fluorescent derivates. This protein is intended to be used as a positive control for enzyme-dependent substrate hydrolysis and for use on immunoblots. Other uses include kinetic studies with novel substrates or identification of inhibitors of beta-lactamase as part of an antimicrobial program. 1165 micromoles of penicillin G hydrolyzed per minute per milligram of total protein.
Specifications
Specifications
| Accession Number | Q9HCU0 |
| Concentration | See Label |
| For Use With (Application) | Kinase Assay |
| Formulation | 200mM potassium phosphate with 50% glycerol and no preservative; pH 7 |
| Gene ID (Entrez) | 57124 |
| Molecular Weight (g/mol) | 38 kDa |
| Name | Beta-Lactamase (TEM-1) |
| pH Range | 7 |
| Purification Method | Purified |
| Quantity | 10 μg |
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