Invitrogen™ Bi-1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143940

Catalog No. PIPA5143940

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human RT4 whole cell, human CACO-2 whole cell. IHC: human endometrial adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue. ICC/IF: Hela cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress-causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, itis thought to involve regulation of Ca2+ handling by the ER.

Specifications

• Antigen: Bi-1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TMBIM6
• Gene Alias: 5031406P05Rik; Ab1-011; Ac1-149; Bax inhibitor 1; Bax inhibitor-1; BAXI1; Bi1; BI-1; Cc1-27; Tegt; testis enhanced gene transcript; testis-enhanced gene transcript protein; Tmbim6; transmembrane BAX inhibitor motif containing 6; transmembrane BAX inhibitor motif-containing protein 6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7009
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P55061
• Gene Symbols: TMBIM6
• Immunogen: A synthetic peptide corresponding to a sequence of human BI-1/TMBIM6 (RKINFDALLKFSHITPSTQQHLK).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG