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Tocris Bioscience™ BI dihydrochloride BI hydrochloride BI hydrochloride
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Catalog No. 746610
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74-661-0 10 mg
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DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers

BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (KD values are 51 and 305 nM, respectively).

Specifications

Chemical Name or Material (5 Z)-3-(1 H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4 H-imidazol-4-one dihydrochloride
Quantity 10 mg
Target Light-up Aptamers
Molecular Formula C19H14F2N4O20.2 HCl
Purity 0.98
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