Invitrogen™ BID Monoclonal Antibody (3C5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA517034

Catalog No. PIMA517034

Description

MA5-17034 targets BID in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17034 immunogen is purified recombinant fragment of human BID expressed in E. Coli. MA5-17034 detects BID which has a predicted molecular weight of approximately 22kDa.

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

Specifications

• Antigen: BID
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.03% sodium azide
• Gene Accession No.: P55957
• Gene Symbols: BID
• Immunogen: Purified recombinant full length human BID protein expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 637
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 3C5
• Conjugate: Unconjugated
• Gene: BID
• Gene Alias: 2700049M22Rik; AI875481; apoptic d; apoptic death agonist; apoptotic death agonist BID; AU022477; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; Bid; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; FP497; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1