Invitrogen™ Human Big ET-1 ELISA Kit

Description

ELISA

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P05305
• Assay Range: 0.4 to 25 pg/mL
• Assay Sensitivity: 0.185 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1906
• Gene Symbol: EDN1
• Interassay CV: 4.9%
• Intraassay CV: 4.7
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Endothelin 1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs