MilliporeSigma™ Bikunin (CP6), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABT1346MI	100 μL	Unconjugated

Catalog No. ABT1346MI Supplier MilliporeSigma™ Supplier No. ABT1346

Description

Rabbit Polyclonal Antibody

Protein AMBP (UniProt P02760; also known as alpha-1-microglobulin/bikunin precursor) is transcribed from the AMBP (also known as HCP, HI30, IATIL, ITIL, ITILC) gene (Gene ID 259) in human as a precursor protein with a signal peptide (a.a. 1-19), posttranslational cleavages produce Alpha-1-microglobulin (a.a. 20-203; A1M, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Protein HC), bikunin (a.a. 206-352; Inter-alpha-trypsin inhibitor light chain, ITI-LC, EDC1, HI-30, Uronic-acid-rich protein), and Trypstatin (a.a. 284-344). Bikunin is a proteoglycan (PG) that contains a single chondroitin sulfate (CS) glycosaminoglycan (GAG) chain with 27-39 monosaccharides attached to Ser10 of the mature core protein. Bikunin GAG has a non-sulfated domain consisting of 6-22 monosaccharides on the non-reducing end and a sulfated domain of 12-18 monosaccharides on the reducing end, with the sulfation occurring exclusively at the fourth carbon of the GalNAc residues. During the synthesis of bikunin by hepatocytes, C-terminal aspartate residues of ∽80kDa heavy chains (HC1 and HC2) are covalently linked to the sixth carbon of a GlcUA residue on the non-sulfated region of the CS chain, forming pre-inter-α-inhibitor (pre-IαI or PαI) with one HC. A second HC can be attached to the non-sulfated region of the CS to form IαI. IαI is known for its roles in inhibiting serine proteinases, such as plasmin, and as an HC donor for the covalent modification of hyaluronan (HA) to form HC·HA (or SHAP·HA) complexes at sites of inflammation and around the oocyte prior to ovulation. Tumor necrosis factor-stimulated gene-6 (TSG-6) forms a covalent HC·TSG-6 intermediate and subsequently transfers the HC to the sixth carbon of GlcNAc residues of HA in a transesterification reaction that contributes to the cross-linking and stabilization of HA matrices; in some contexts modification of HA with HC has been implicated in the promotion of leukocyte adhesion. In addition, the bikunin·CS chain can accept a single HC from IαI via transfer catalyzed by TSG-6 and that HCs could be swapped from bikunin·CS chain to HA. (Briggs, D.C., et al. (2015). J. Biol. Chem. 290(48):28708-28723).

Specifications

• Antigen: Bikunin (CP6)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene: AMBP, HCP, HI30, IATIL, ITIL, ITILC
• Gene Accession No.: P02760
• Host Species: Rabbit
• Immunogen: Diphtheria toxoid-conjugated linear peptide corresponding to human bikunin N-terminal sequence.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001624
• Test Specificity: This rabbit polyclonal antiserum targets Inter-alpha-trypsin inhibitor light chain (a.a. 206-352; ITI-LC, Bikunin, EDC1, HI-30, Uronic-acid-rich protein) N-terminal sequence, it will also recognize the precursor protein AMBP (a.a. 1-352; alpha-1-microglobulin/bikunin precursor), but not the other two AMBP-derived products, Alpha-1-microglobulin (a.a. 20-203; A1M, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Protein HC) and Trypstatin (a.a. 284-344).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.