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R&D Systems™ Biotin-PEG4-Diazirine
SDP

Catalog No. RD10188009 Shop All Novus Products
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RD10188009 5 mg
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Catalog No. RD10188009 Supplier R&D Systems™ Supplier No. 8994/5

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Description

A Tocris product, part of R&D Systems. Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle

Key information:Biotin-PEG4-Diazirine is a cell-permeable photo-crosslinking probe for elucidating protein interactome of polyarginine cell-penetrating peptides and mapping cell surfaceome through deazaflavin-diazirine energy-transfer labelling (DarT labelling). Upon UV irradiation and in the presence of deazaflavin as an organic photocatalyst, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby proteins. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. Suitable for live-cell applications.

Used for: photocatalytic proximity labelling.

Application: deazaflavin-diazirine energy-transfer labelling (DarT labelling) followed by affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures.

Specifications

Chemical Name or Material 1-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)-N-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)-3,6,9,12-tetraoxapentadecan-15-amide
Quantity 5 mg
Product Line Tocris
Molecular Formula C30H43F3N6O7S
Purity 95%
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