Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R), Novus Biologicals™

Rabbit Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP26139220	Novus Biologicals NBP26139220UG	20 μg
NB261392100	Novus Biologicals NBP261392100UG	100 μg
NB261392200	Novus Biologicals NBP261392200UG	200 μg

Description

Biotin (Vitamin B7 or Vitamin H) Monoclonal specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization.

Specifications

• Antigen: Biotin (Vitamin B7 or Vitamin H)
• Dilution: Western Blot, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
• Classification: Monoclonal
• Form: Purified
• Target Species: All species
• Immunogen: Biotinylated sheep immunoglobulin
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 2.4 kDa

• Clone: BTN/2032R
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
• Conjugate: Unconjugated
• Host Species: Mouse
• Formulation: Prepared in 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
• Isotype: IgG1
• Purification Method: Protein A or G purified
• Test Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.