BioTracker 543 Yellow β-Gal Dye, MilliporeSigma™

Description

Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).

• β-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).
• The BioTracker 543 Yellow β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of β-galactosidase, this probe exhibits good S/N ratio.
• Absorbance: 525 nm (488 nm compatible)
• Emission: 543 nm

Specifications

• Absorbance: 525 nm
• Quantity: 10 x 50 μg