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BL21-lysate Rabbit anti-Bacteria, Polyclonal, Proteintech
SDP

Catalog No. 501726404 Shop All Proteintech Group Inc Products
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Catalog No. 50-172-6404 Supplier Proteintech Group Inc Supplier No. 105571IG150UL
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Rabbit Polyclonal Antibody

Escherichia coli B serves as a research model for studying phage sensitivity, restriction-modification systems, and bacterial evolution, and also as a workhorse for protein expression in life science laboratories and in the biotech industry. Characteristics such as protease deficiency, low acetate production at a high level of glucose, and enhanced permeability (probably due to a simple cell surface) make E. coli B a desirable host for the production of genetically engineered proteins. BL21(DE3) has been very widely used to express recombit proteins. Comparison of BL21(DE3) and another B strain REL606 shows they differ in length by 72. 304 bp and have 426 single nucleotide polymorphisms, all of which have been accounted for by various laboratory manipulations. Differences between B strains and K12 include the absence of flagellar component genes, the DNA cytosine methylase dcm, and ompT in BL21(DE3). B strains may have an additional type II secretion system not found in K12. Other differences include differential distribution of insertions sequences and other changes resulting from horizontal gene transfer. BL21(DE3) also carries a DE3 recombit phage harboring the T7 RNA polymerase gene that can direct high-level expression of cloned genes under the control of the T7 promoter.

Specifications

Antigen BL21-lysate
Applications Western Blot
Classification Polyclonal
Concentration 0.7 mg/mL
Conjugate Unconjugated
Formulation PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
Gene Alias BL21 lysate
Host Species Rabbit
Immunogen BL21-lysate Fusion Protein Ag0049
Purification Method Protein A
Quantity 150 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Bacteria
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
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