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Invitrogen™ Blimp-1 Monoclonal Antibody (3H2-E8), HRP

Description
In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining. Suggested positive control: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.
Specifications
Specifications
| Antigen | Blimp-1 |
| Applications | ChIP Assay, ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry |
| Classification | Monoclonal |
| Clone | 3H2-E8 |
| Concentration | 0.6 mg/mL |
| Conjugate | HRP |
| Formulation | PBS with no preservative |
| Gene | PRDM1 |
| Gene Accession No. | O75626, Q60636 |
| Gene Alias | B lymphocyte-induced maturation protein 1; b2b1765Clo; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; Blimp1; BLIMP-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR/SET domain 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; Prdm1; RP1-134E15.1; ZNFPR1A1 |
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