Invitrogen™ Blood Group Lewis A Monoclonal Antibody (7LE)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119344

Catalog No. 01-676-100

Description

This antibody recognizes Lewis^a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewis^a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors. For IHC (P), heat mediated antigen retrieval in sodium citrate is recommended.

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

Specifications

• Antigen: Blood Group Lewis A
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15 mM sodium azide; pH 7.4
• Gene Accession No.: P21217
• Gene Symbols: FUT3
• Immunogen: Mucin from ovarian cyst fluid.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 7LE
• Conjugate: Unconjugated
• Gene: FUT3
• Gene Alias: 4-fucosyltransferase; alpha-(1,3/1,4)-fucosyltransferase; blood group Lewis alpha-4-fucosyltransferase; CD174; FT3B; Fucosyltransferase 3; fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group); fucosyltransferase 3 (Lewis blood group); fucosyltransferase III; FucT-III; FUT3; Galactoside 3(4)-L-fucosyltransferase; LE; Les; Lewis FT; Lewis Y
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 2525
• Content And Storage: 4°C, do not freeze
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov