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Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media

Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases

Supplier:  Cytiva 17094801

Catalog No. 45-002-968

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This medium is well established as adsorbent for albumin and interferon at laboratory and process scale.

  • Cibacron Blue 3G is covalently coupled to the base matrix allowing for low ligand leakage and high capacity
  • The blue dye binds proteins, such as albumin, interferon, lipoproteins and blood coagulation factors
  • The dye also binds enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+
  • Avg. particle size: 90μm
  • Volume: 50mL

Developed and tested in co-operation with large-scale manufacturers and is used in routine commercial production.

  • For large-scale purification of interferon and albumin
  • For isolating groups of enzymes and removing of albumin
  • For industrial applications and other approved applications


Certificate of Analysis



50 mL
Stable in commonly used aqueous Buffers: 70% Ethanol, 6 M Guanidine Hydrochloric, 8 M Urea
Blue Sepharose 6 Fast Flow Media
45 to 165 μm
25 cm
100 kPa
4 to 12
Cibacron Blue
For Albumin and Interferon at Both Laboratory and Process Scale
2°C to 8°C
250 to 400 cm/Hoμr
9.84 in.
Certificate of Analysis
6% Cross-Linked Agarose
20% Ethanol, 0.1 M Potassium Phosphate Buffer




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