MilliporeSigma™ BMAL1, Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABE2627MI	100 μg	Unconjugated

Catalog No. ABE2627MI Supplier MilliporeSigma™ Supplier No. ABE2627

Description

Rabbit Polyclonal Antibody

Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: O00327 also known as Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, BMAL1, Class E basic helix-loop-helix protein 5, bHLHe5; Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3) is encoded by the ARNTL (also known as BHLHE5; BMAL1; MOP3; PASD3) gene (gene ID 406) in human. BMLA1 is highly expressed in the adult brain, skeletal muscle, and heart. It is a transcriptional activator that forms a core component of the circadian clock. These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop. They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes. PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop. They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity. BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. BMAL1 is shown to positively regulate myogenesis and negatively regulate adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. BMAL1 is the only component of the mammalian circadian clock whose deletion in a murine model is shown to generate arrhythmicity. BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and they age quickly.

Specifications

• Antigen: BMAL1
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: ARNTL, BHLHE5, BMAL1, MOP3, PASD3
• Gene Accession No.: O00327
• Host Species: Rabbit
• Immunogen: KLH-conjugated peptide corresponding to the C-terminal 20 amino acids of human BMAL1 protein.
• Purification Method: Affinity Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001284648
• Test Specificity: Detects BMAL1 (Aryl hydrocarbon receptor nuclear translocator-like protein 1) in HeLa cell nuclear extract.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.