BMP-2 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P195100	Invitrogen™ 500P195100UG	100 μg

Description

AA Sequence of recombinant protein: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BMP-2. Anti-Human/Murine/Rat BMP-2-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BMP-2 matrix. Sandwich ELISA: To detect Human/Murine/Rat BMP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human/Murine/Rat BMP-2 (500-P195Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human/Murine/Rat BMP-2. Western Blot: To detect Human/Murine/Rat BMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human/Murine/Rat BMP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (Hogan 1996, Reddi 1998 and Francis-West et al. 1999). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration. Lovostatin increases bone formation by turning on the bmp-2 gene (Mundy et al. 1999). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (Macias et al. 1999, Lecanda et al. 1997). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (Hogan 1996, Dale and Wardle 1999). BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (Massague 1994, Derynck 1997, Attisano 1993).

Specifications

• Antigen: BMP-2
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human, Mouse, Rat
• Gene Accession No.: P12643, P21274, P49001
• Gene ID (Entrez): 12156, 29373, 650
• Immunogen: E.coli-derived Recombinant Human/Murine/Rat BMP-2.
• Purification Method: Antigen Affinity Chromatography
• Gene: BMP2

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 2610024H22Rik; AI467020; AL117858; AW546137; BB189135; BDA2; BMP; BMP2; BMP-2; BMP2A; BMP-2A; BMPRII; BMPR-II; Bone morphogenetic; Bone morphogenetic protein; Bone morphogenetic protein 2; bone morphogenetic protein 2 precursor (BMP-2) (BMP-2A); bone morphogenetic protein 2A; H-BMP-2; morphogen
• Gene Symbols: BMP2
• Primary or Secondary: Primary
• Content And Storage: -20°C