Invitrogen™ BOB-1 Monoclonal Antibody (SP92)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA516323

Catalog No. PIMA516323

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Specifications

• Antigen: BOB-1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: POU2AF1
• Gene Alias: B-cell-specific coactivator OBF-1; BOB.1; Bob1; BOB-1; OBF.1; OBF1; Obf-1; OCAB; OCA-B; OCT-binding factor 1; POU class 2 associating factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1; Pou2af1
• Host Species: Rabbit
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 5450
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: SP92
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: Q16633
• Gene Symbols: POU2AF1
• Immunogen: Synthetic peptide corresponding to C-terminus of human BOB-1 protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG