Invitrogen™ Bora Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA589480

Catalog No. PIPA589480

Description

Immunogen sequence: KYSLGSITSP SPISSPTFSP IEFQIGETPL SEQRKFTVHS PDASSGTNSN GITNPCIRSP YIDGCSPIKN WSPMRLQMYS GGTQYRTSVI QIPFTLETQG EDEEDKENIP STDVSSPAMD AAGIHLRQFS NEASTHGTHL VVTAMSVTQN QSSASEKELA LLQDVEREKD NNTVDMVDPI EIADETTWIK EPVDNGSLPM TDFVSGIAFS IENSHMCMSP LAESSVIPCE SSNIQMDSGY NTQNCGSNIM DTVGAESYCK ESDAQTCEVE SKSQAFNMKQ DHTTQRCWMK TASPFQCSSP; Positive Samples: HT-29, HeLa.

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Specifications

• Antigen: Bora
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BORA
• Gene Alias: 6720463M24Rik; AI317232; aurora borealis; BORA; bora, aurora kinase A activator; C13orf34; HsBora; Protein aurora borealis; RGD1309522; RP11-342J4.2
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79866
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.97 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q6PGQ7
• Gene Symbols: BORA
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-559 of human BORA (NP_079084.4).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG