Invitrogen™ Bora Recombinant Rabbit Monoclonal Antibody (10H2L1)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 702148

Catalog No. PI702148

Description

This antibody is predicted to react with Monkey, Horse, Bovine and Sheep Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Specifications

• Antigen: Bora
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q5M864, Q6PGQ7, Q8BS90
• Gene Symbols: BORA
• Immunogen: Protein corresponding to Human BORA (aa 274-456).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: 10H2L1
• Conjugate: Unconjugated
• Gene: BORA
• Gene Alias: 6720463M24Rik; AI317232; aurora borealis; BORA; bora, aurora kinase A activator; C13orf34; HsBora; Protein aurora borealis; RGD1309522; RP11-342J4.2
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 306102, 77744, 79866
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid