Invitrogen™ Brachyury Monoclonal Antibody (X1AO2), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 14977082

Catalog No. 50-112-2852

Description

Description: The monoclonal antibody X1AO2 recognizes human Brachyury, a 47 kDa protein encoded by the T gene and functioning as a nuclear transcription factor. This transcription factor binds to specific DNA regions near the palindromic sequence TCACACCT (T-site) through a region in its N-terminus, called the T-box. Brachyury functions during embryogenesis to regulate midline development by establishing the anterior/posterior axis through the regulation of genes involved in mesoderm formation and differentiation. Brachyury is the primary mesoderm marker used to distinguish differentiation of embryonic stem cells into a mesenchymal stem cell lineage. Variations in the T gene in humans are associated with neural tube defects and chordomas. Expression of brachyury is also increased in advanced stage lung tumors and tumor cell lines. Applications Reported: This X1AO2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This X1AO2 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL. This X1AO2 antibody has also been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 5 μg/mL.

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Specifications

• Antigen: Brachyury
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O15178
• Gene Symbols: TBXT
• Immunogen: E.coli-derived fragment of human Brachyury corresponding to amino acids 237-348.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: X1AO2
• Conjugate: Unconjugated
• Gene: TBXT
• Gene Alias: Bra; brachyury; brachyury homolog; brachyury protein; brachyury-like 2; brachyury-like 3; cou; D17Mit170; Low; low ratio; Lr; me75; Protein T; RP11-459F1.1; SAVA; T; T brachyury homolog; T brachyury transcription factor; T, brachyury homolog; T1; T-box transcription factor T; TBXT; TFT; Tl2; Tl3
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6862
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.