Invitrogen™ BRCA1 Monoclonal Antibody (17F8)

Catalog No.	Quantity
AMPB3160	100 μL

Catalog No. AMPB3160 Supplier Invitrogen™ Supplier No. MA123160

Description

Mouse Monoclonal Antibody

Recommended positive controls: 293T, A431, HeLa. This antibody does not recognize the delta exon 11 splice variant of BRCA1. The antigen used to product MA1-23160 has a predicted molecular weight of ~208 kDa.

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Specifications

• Antigen: BRCA1
• Applications: ChIP Assay, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 17F8
• Concentration: 1.55 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: BRCA1
• Gene Accession No.: P38398, P48754
• Gene Alias: brca 1; Brca1; brca-1; BRCA1, DNA repair associated; BRCA1/BRCA2-containing complex, subunit 1; BRCAI; BRCC1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; breast cancer associated 1; breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; BROVCA1; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; FANCS; IRIS; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; PNCA4; PPP1R53; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; RNF53; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein
• Gene Symbols: BRCA1
• Host Species: Mouse
• Immunogen: Protein fragment expressed in E. coli corresponding to amino acids 762-1315.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12189, 672
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1